ZnHA/TiO₂复合涂层的制备及生物相容性

来源期刊：金属学报2011年第4期

论文作者：张静莹 齐民 杨大颐 艾红军

文章页码：429 - 434

关键词：Zn； Ti； TiO₂； 羟基磷灰石； 成骨细胞； 生物相容性

Key words：Zn； Ti； TiO₂； hydroxyapatite； osteoblast； biocompatibility

摘    要：为改善羟基磷灰石(HA)涂层与Ti基体的结合性能及成骨性能,在纯Ti表面先通过微弧氧化方法形成多孔TiO₂,然后通过溶胶凝胶法在其上形成ZnHA生物涂层.利用SEM和XRD观察和分析样品的表面形貌和物相组成,采用火焰原子吸收分光光度法测量Zn²⁺的释放浓度.将MG-63细胞直接接种于样品表面,评价成骨细胞在样品表面的黏附和增殖.噻唑蓝(MTT)比色分析结果表明,细胞在ZnHA/TiO₂-2和ZnHA/TiO₂-3涂层的样品表面增殖率比对照组(HA)显著增加,倒置荧光显微镜下可观察到细胞在改性样品表面黏附和伸展良好.由此表明,带有ZnHA/TiO₂复合涂层的Ti种植体应具有更好的机械结合性能和生物相容性.

Abstract: To improve the binding and osteogenic properties for the hydroxyapatite (HA) coating on Ti substrate,a ZnHA biological coating was prepared on commercially pure Ti by sol-gel method after a treatment by micro-arc oxidation (MAO) to produce an porous titania (TiO₂) coating on Ti. The morphologies and phases components of the coating were characterized by SEM and XRD.The release concentration of Zn²⁺ was measured by flame atomic absorption spectrometry.In vitro coculture assays were performed to evaluate the cell proliferation and adhesion of MG-63 cells to the coated Ti.The proliferation rates of the cells were found to be significantly higher on the ZnHA/TiO₂- 2 and ZnHA/TiO₂-3 coatings than that of the control group(HA) by MTT.It was confirmed by the fluorescence microscopy observations that the osteoblast-like cells on the hybrid coating were favorably adhered and spread.The results suggest that the ZnHA/TiO₂ hybrid coatings on Ti implants should possess better binding property and biocompatibility.