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of SYBR Green Real- time PCR Master Mix (Toyobo Co., Ltd., Osaka, Japan), 1 μL of 10 mmol/L forward and reverse primer, 2 μL of genomic DNA, and 8.5 μL of nuclease-free water. The real-time PCR was carried......
are unstable and easily lost, making their industrial use difficult. To overcome these hurdles, metabolically diverse acidophilic microorganisms have been explored in the construction of various co-culture... and sessile cells were collected as previously described in Ref. [22]. DNA from planktonic, sessile, and total cells was extracted using a TIANamp genomic DNA purification kit (Tiangen Biotech, Co., Ltd......
), hydrogen peroxide (H2O2) and potassium dichromate (K2Cr2O7) were purchased from Sinopharm Chemical Reagent Co., Ltd. The stock solution of Cr(VI) was prepared by dissolving K2Cr2O7 in ultrapure......
； 2. School of Civil Engineering, Central South University, Changsha 410075, China； 3. China Construction Tunnel Co. Ltd., Chongqing, 401320, China) Abstract: There are a lot of cases that shield......
and also to remove the residual water and alcohol. In order to avoid cracking, the coated samples were initially held at 60 °C in a digital oven (Pars Azma Co.) for 2 h......
Metallurgical Quarterly, 2001, 40(4): 395-420. [14] CUI J, DU Y G, XIAO H X, YI Q S, DU D Y. A new process of continuous three-stage co-precipitation of arsenic with ferrous iron and lime [J... with iron(III) in aqueous sulfate media: Effect of time, lime as base and co-ions on arsenic retention [J]. Water Research, 2008, 42(3): 661-668. [25] MONTGOMERY D C. Design and analysis of experiments [M......
, Jiangxi University of Science and Technology, Ganzhou 341000, China; 3. Mining Research Laboratory, Jiangxi Ionic Rare Earth Engineering Research Co., Ltd., Ganzhou 341000, China; 4. National......
(Group) Co., Ltd., Chongqing 400054, China Central South University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract: Due to the current trend towards lightweight design......
. China Nerin Engineering Co., Ltd., Nanchang 330002, China) Abstract: In order to clarify the enhanced stirring mechanism of oscillatory gas jet in bottom-blown bath, a new way based on Betti numbers......
J. Cent. South Univ. (2020) 27: 2017-2031 DOI: https://doi.org/10.1007/s11771-020-4427-6 Isothermal diffusion of water vapor in unsaturated soils based on Fick’s second law LIU Fei-fei(刘飞飞)1, MAO Xue-song(毛雪松)1, ZHANG Jian-xun(张建勋)1,WU Qian(吴谦)1, LI Ying-ying(李颖颖)1, 2, XU Cheng(许铖)1 1. School of Highway, Chang’an University, Xi’an 710064, China; 2. Luoyang Rail Transit Group Co., Ltd., Luoyang......
