In vitro Biological Effects of Ti2448 Alloy Modified by Micro-arc Oxidation and Alkali Heatment

Xue Han 1,2),Hongchen Liu 1),Dongsheng Wang 1),Shujun Li 3),Rui Yang 3),Xiaojie Tao 3) and Xiaohong Jiang 4) 1) Dental Institute,General Hospital of the Chinese People s Liberation Army,Beijing 100853,China 2) Department of Stomatology,General Hospital of Shenyang Military Region,Shenyang 110015,China 3) Shenyang National Laboratory for Materials Science,Institute of Metal Research,Chinese Academy of Sciences,Shenyang 110016,China 4) Department of Orthodontics,Shenyang Hospital of Stomatology,Shenyang 110002,China

摘 要：The purpose of this study was to test the hypothesis that the combination of micro-arc oxidation and alkali heatment （MAH） would improve the cytocompatibility of a newly designed Ti-24Nb-4Zr-8Sn alloy.In this study,commercially pure titanium （cp Ti） and Ti-24Nb-4Zr-8Sn were used.Surface modification of Ti-24Nb4Zr-8Sn by a two-step treatment of micro-arc oxidation （MAO） and alkali heatment was reported.Surface characterizations were performed by scanning electron microscopy （SEM）,thin film X-ray diffraction （TF-XRD） and X-ray photoelectron spectroscopy （XPS）.The MAH layer consisted of finer crystals and possessed a higher degree of crystallity and stability than the MAO layer.A biocompatibility study on treated and untreated Ti24Nb-4Zr-8Sn in comparison with cp Ti was carried out to investigate the effect of the different surfaces on the bone integration property in vitro.The cellular assays revealed that the MAO and MAH layer favored the initial adhesion of MC3T3-E1 cells and that the growth rate of MC3T3-E1 cells on MAH layer was significantly higher than that on the conventional MAO-treated layer after 3-day and 5-day incubation,demonstrating the greater potential of the hybrid treatment of micro-arc oxidation followed with alkali heatment as a novel surface modification method for implanting materials.

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