Anti-inflammatory Effects and Mechanisms of Usnic Acid
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2011年第5期
论文作者：黄志军 阮金兰
文章页码：955 - 959
摘 要：The anti-inflammatory effect and mechanism of Usnic acid （UA） were explored on lipopoly-saccharide （LPS）-stimulated RAW264.7 cell line.The effects of UA on pro-inflammatory cytokines including tumor necrosis factor-alfa （TNF-α）,interleukin-6 （IL-6） and interleukin-1 beta （IL-1β）,pro-inflammatory mediators such as nitric oxide （NO）,inducible nitric oxide synthase （iNOS） and cyclooxygenase-2 （COX-2） were studied by sandwich ELISA,real-time PCR and western blot analyses.Similarly,the effect of UA on anti-inflammatory cytokine interleukin-10 （IL-10） and anti-inflammatory mediator heme oxygenase-1 （HO-1） were also studied following the same methods.Furthermore,nuclear factor-κB （NF-κB） was assayed by immunocytochemistry.The results showed that UA has anti-inflammatory effect by down-regulatinng iNOS,COX-2,IL-1β,IL-6 and TNF-α,COX-2 gene expression through the suppression of NF-κB activation and increasing anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1 production.
黄志军1,2，阮金兰1
1. College of Pharmacy,Tongji Medical Center,Huazhong University of Science and Technology2. School of Chemical Engineering,Wuhan University of Technology
摘 要：The anti-inflammatory effect and mechanism of Usnic acid （UA） were explored on lipopoly-saccharide （LPS）-stimulated RAW264.7 cell line.The effects of UA on pro-inflammatory cytokines including tumor necrosis factor-alfa （TNF-α）,interleukin-6 （IL-6） and interleukin-1 beta （IL-1β）,pro-inflammatory mediators such as nitric oxide （NO）,inducible nitric oxide synthase （iNOS） and cyclooxygenase-2 （COX-2） were studied by sandwich ELISA,real-time PCR and western blot analyses.Similarly,the effect of UA on anti-inflammatory cytokine interleukin-10 （IL-10） and anti-inflammatory mediator heme oxygenase-1 （HO-1） were also studied following the same methods.Furthermore,nuclear factor-κB （NF-κB） was assayed by immunocytochemistry.The results showed that UA has anti-inflammatory effect by down-regulatinng iNOS,COX-2,IL-1β,IL-6 and TNF-α,COX-2 gene expression through the suppression of NF-κB activation and increasing anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1 production.
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