Novel synthesis method combining a foaming agent with freeze-drying to obtain hybrid highly macroporous bone scaffolds
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2020年第8期
论文作者：Paulina Kazimierczak AleksANDra Benko Krzysztof Palka Cristina Canal Dorota Kolodynska Agata Przekora
文章页码：52 - 63
摘 要：Three-dimensional macroporous scaffolds are commonly used in bone tissue engineering applications since they provide sufficient space for cell migration and proliferation, facilitating bone ingrowth and implant vascularisation. The aim of this work was to combine two simple methods, freeze-drying and gas-foaming, in order to fabricate highly macroporous bone scaffolds made of chitosan/agarose matrix reinforced with nanohydroxyapatite. The secondary goal of this research was to comprehensively assess biomedical potential of developed biomaterials. In this work, it was demonstrated that simultaneous application of freeze-drying and gas-foaming technique allows to obtain hybrid（as proven by ATR-FTIR）macroporous bone scaffolds（pore diameter > 50 um） characterized by high open（70%） and interconnected porosity. Novel scaffolds were non-toxic, favoured osteoblasts adhesion and growth and induced apatite formation on their surfaces, indicating their high bioactivity that is essential for good implant osseointegration. Biomaterials were also prone to enzymatic degradation, degradation in acidified microenvironment（e.g. osteoclast-mediated）, and slow degradation under physiological p H of 7.4.Moreover, the scaffolds revealed microstructure（70% open porosity, SSA approx. 30 m2/g, high share of macropores with diameter in the range 100-410 um） and compressive strength（1–1.4 MPa） comparable to cancellous bone, indicating that they are promising implants for cancellous bone regeneration.
Paulina Kazimierczak1，AleksANDra Benko2，Krzysztof Palka3，Cristina Canal4,5，Dorota Kolodynska6，Agata Przekora1
1. Department of Biochemistry and Biotechnology, Medical University of Lublin2. AGH University of Science and Technology, Faculty of Materials Science and Ceramics3. Department of Materials Engineering, Lublin University of Technology4. Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Universitat Politècnica de Catalunya5. Research Centre in Multiscale Science and Engineering, Universitat Politècnica de Catalunya6. Department of Inorganic Chemistry, Maria Curie-Sklodowska University
摘 要：Three-dimensional macroporous scaffolds are commonly used in bone tissue engineering applications since they provide sufficient space for cell migration and proliferation, facilitating bone ingrowth and implant vascularisation. The aim of this work was to combine two simple methods, freeze-drying and gas-foaming, in order to fabricate highly macroporous bone scaffolds made of chitosan/agarose matrix reinforced with nanohydroxyapatite. The secondary goal of this research was to comprehensively assess biomedical potential of developed biomaterials. In this work, it was demonstrated that simultaneous application of freeze-drying and gas-foaming technique allows to obtain hybrid（as proven by ATR-FTIR）macroporous bone scaffolds（pore diameter > 50 um） characterized by high open（70%） and interconnected porosity. Novel scaffolds were non-toxic, favoured osteoblasts adhesion and growth and induced apatite formation on their surfaces, indicating their high bioactivity that is essential for good implant osseointegration. Biomaterials were also prone to enzymatic degradation, degradation in acidified microenvironment（e.g. osteoclast-mediated）, and slow degradation under physiological p H of 7.4.Moreover, the scaffolds revealed microstructure（70% open porosity, SSA approx. 30 m2/g, high share of macropores with diameter in the range 100-410 um） and compressive strength（1–1.4 MPa） comparable to cancellous bone, indicating that they are promising implants for cancellous bone regeneration.
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