Surface energy-mediated fibronectin adsorption and osteoblast responses on nanostructured diamond
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2019年第5期
论文作者：Yixing Tian Huiling Liu Brian W.Sheldon Thomas J.Webster Sichen Yang Huilin Yang Lei Yang
文章页码：817 - 823
摘 要：Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influence to protein adsorption and subsequent cellular responses along with the mechanism behind such capacity becomes more important. Here the role of surface energy associated with nanostructured diamond in modulating fibronectin and osteoblast（OB, bone forming cells） responses was investigated. Nanocrystalline diamond（NCD） and submicron crystalline diamond（SMCD） films with controllable surface energy were prepared by microwave-enhanced plasma chemical vapor deposition（MPCVD） techniques. Fibronectin adsorption on the diamond films with varied surface energy values was measured via the enzyme-linked immunosorbent assay（ELISA） and the relationship between the surface energy and fibronectin adsorption was studied. The result indicated that fibronectin adsorption on nanostructured surfaces was closely related to both surface energy and material microstructures. The spreading and migration of OB aggregates（each containing 30–50 cells） on the NCD with varied surface energy values were also studied. The result indicates a correlation between the cell spreading and migration on nanodiamond and the surface energy of nanostructured surface.
Yixing Tian1，Huiling Liu1，Brian W.Sheldon2，Thomas J.Webster3,4，Sichen Yang5，Huilin Yang1,4，Lei Yang1,4
1. Orthopaedic Institute and Department of Orthopaedics, The First Affiliated Hospital, Soochow University2. School of Engineering, Brown University3. Department of Chemical Engineering, Northeastern University4. International Research Center for Translational Orthopaedics (IRCTO), Soochow University5. UWC Changshu China
摘 要：Nanostructured diamond have potential applications in many biomedical related fields and demonstrated extraordinary capacity to influence cellular responses. Studying the surface property of nanodiamond and its influence to protein adsorption and subsequent cellular responses along with the mechanism behind such capacity becomes more important. Here the role of surface energy associated with nanostructured diamond in modulating fibronectin and osteoblast（OB, bone forming cells） responses was investigated. Nanocrystalline diamond（NCD） and submicron crystalline diamond（SMCD） films with controllable surface energy were prepared by microwave-enhanced plasma chemical vapor deposition（MPCVD） techniques. Fibronectin adsorption on the diamond films with varied surface energy values was measured via the enzyme-linked immunosorbent assay（ELISA） and the relationship between the surface energy and fibronectin adsorption was studied. The result indicated that fibronectin adsorption on nanostructured surfaces was closely related to both surface energy and material microstructures. The spreading and migration of OB aggregates（each containing 30–50 cells） on the NCD with varied surface energy values were also studied. The result indicates a correlation between the cell spreading and migration on nanodiamond and the surface energy of nanostructured surface.
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