Fast Mineralization of Densely Packed Hydroxyapatite Layers in the Presence of Over-expressed Recombinant Amelogenin
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2017年第2期
论文作者：王梦湖 XIE Hao XIE Jingjing PING Hang TAN Tiening JI Wei 傅正义
文章页码：256 - 263
摘 要：Learning from the process of biominerals formation provides tremendous ideas for developing advanced synthesis techniques. According to the structure formation of tooth enamel, a recombinant amelogenin his-Amel X was designed and constructed. The protein was over-expressed and could be conveniently purified in one-step heat treatment. The mineralization process of hydroxyapatite was initiated by enzyme AP and regulated by the recombinant amelogenin. Effects of solution pH value and mineralization duration were studied. It was demonstrated that his-Amel X could induce the nucleation of apatite and quicken the growth rate at pH 7.0-7.4, while impeded hydroxyapatite growth at pH 6.8. Moreover, a much denser layer of hydroxyapatite was achieved with the addition of his-Amel X. The present study may not only provide insight into the formation of natural biomaterials but also open a new path to prepare materials under environmentally benign conditions.
王梦湖1，XIE Hao2，XIE Jingjing1，PING Hang1，TAN Tiening1，JI Wei1，傅正义1
1. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology2. School of Science,Wuhan University of Technology
摘 要：Learning from the process of biominerals formation provides tremendous ideas for developing advanced synthesis techniques. According to the structure formation of tooth enamel, a recombinant amelogenin his-Amel X was designed and constructed. The protein was over-expressed and could be conveniently purified in one-step heat treatment. The mineralization process of hydroxyapatite was initiated by enzyme AP and regulated by the recombinant amelogenin. Effects of solution pH value and mineralization duration were studied. It was demonstrated that his-Amel X could induce the nucleation of apatite and quicken the growth rate at pH 7.0-7.4, while impeded hydroxyapatite growth at pH 6.8. Moreover, a much denser layer of hydroxyapatite was achieved with the addition of his-Amel X. The present study may not only provide insight into the formation of natural biomaterials but also open a new path to prepare materials under environmentally benign conditions.
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