Effect of yttrium ion on the proliferation,differentiation and mineralization function of primary mouse osteoblasts in vitro
来源期刊：JOURNAL OF RARE EARTHS2010年第3期
论文作者：张金超 刘翠莲 李亚平 孙静 王鹏 邸科前 陈航 赵燕燕
文章页码：466 - 470
摘 要：A series of experimental methods including MTT test,alkaline phosphatase（ALP） activity measurement,oil red O stain and measurement and mineralized function were employed to assess the effects of Y3+ on the proliferation,differentiation,adipogenic transdifferentiation and mineralization function of primary mouse osteoblasts（OBs） in vitro.The results indicated that Y3+（1×10-9,1×10-8,1×10-7,1×10-6,1×10-5,and 1×10-4 mol/L） promoted the proliferation of OBs on day 1,2 and 3.Y3+ had no effect on the differentiation of OBs at concentrations of 1×10-9 and 1×10-8 mol/L,promoted the differentiation of OBs at concentration of 1×10-7 mol/L,but turned to inhibit the differentiation of OBs at other tested concentrations on day 1.On day 2,Y3+ inhibited the differentiation of OBs at all tested concentrations.On day 3,Y3+ promoted the differentiation of OBs at lower concentrations of 1×10-9 and 1×10-8 mol/L,but turned to inhibit the differentiation of OBs at other concentrations.Y3+ promoted the adipocytic transdifferentiation of OBs at most tested concentrations on day 9 and 12.On day 15,Y3+ promoted the adipocytic transdifferentiation of OBs at lower concentrations of 1×10-9 and 1×10-8 mol/L,turned to inhibit at other tested concentrations.Y3+ inhibited the formation of mineralized matrix nodules of OBs at concentrations of 1×10-9,1×10-8 and 1×10-6 mol/L,but turned to promote the formation of mineralized matrix nodules of OBs at other tested concentrations.These findings suggested that the effects of Y3+ on the proliferation,differentiation,adipocytic transdifferentiation and mineralization function of primary OBs depended on the concentration and culture time,moreover,concentration and culture time were pivotal factors for switching the biological effects of Y3+ from toxicity to activity,from damage to protection,or from down-regulation to up-regulation.
张金超1，刘翠莲1，李亚平1，孙静2，王鹏2，邸科前3，陈航2，赵燕燕3
1. College of Chemistry & Environmental Science,Chemical Biology Laboratory,Hebei University2. Affiliated Hospital of Hebei University3. Experimental Center,Hebei University
摘 要：A series of experimental methods including MTT test,alkaline phosphatase（ALP） activity measurement,oil red O stain and measurement and mineralized function were employed to assess the effects of Y3+ on the proliferation,differentiation,adipogenic transdifferentiation and mineralization function of primary mouse osteoblasts（OBs） in vitro.The results indicated that Y3+（1×10-9,1×10-8,1×10-7,1×10-6,1×10-5,and 1×10-4 mol/L） promoted the proliferation of OBs on day 1,2 and 3.Y3+ had no effect on the differentiation of OBs at concentrations of 1×10-9 and 1×10-8 mol/L,promoted the differentiation of OBs at concentration of 1×10-7 mol/L,but turned to inhibit the differentiation of OBs at other tested concentrations on day 1.On day 2,Y3+ inhibited the differentiation of OBs at all tested concentrations.On day 3,Y3+ promoted the differentiation of OBs at lower concentrations of 1×10-9 and 1×10-8 mol/L,but turned to inhibit the differentiation of OBs at other concentrations.Y3+ promoted the adipocytic transdifferentiation of OBs at most tested concentrations on day 9 and 12.On day 15,Y3+ promoted the adipocytic transdifferentiation of OBs at lower concentrations of 1×10-9 and 1×10-8 mol/L,turned to inhibit at other tested concentrations.Y3+ inhibited the formation of mineralized matrix nodules of OBs at concentrations of 1×10-9,1×10-8 and 1×10-6 mol/L,but turned to promote the formation of mineralized matrix nodules of OBs at other tested concentrations.These findings suggested that the effects of Y3+ on the proliferation,differentiation,adipocytic transdifferentiation and mineralization function of primary OBs depended on the concentration and culture time,moreover,concentration and culture time were pivotal factors for switching the biological effects of Y3+ from toxicity to activity,from damage to protection,or from down-regulation to up-regulation.
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