Effects of gadolinium on proliferation, differentiation and calcification of primary mouse osteoblasts in vitro
来源期刊：JOURNAL OF RARE EARTHS2012年第8期
论文作者：张金超 李亚平 张群 郝晓红 王书香
文章页码：831 - 834
摘 要：To evaluate the effects of Gd on proliferation, differentiation and mineralization function of primary osteoblasts （OBs） in vitro, we tested cell viability by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay, cell differentiation by alkaline phosphatase （ALP） activity assay, synthesis of type ? collagen, and oil red O and alizarin red S （ARS） stain assays. The results indicated that effects of Gd on the proliferation, osteogenic differentiation, mineralization function and adipocytic transdifferentiation of primary OBs de-pended on concentration and incubation time, but were not dose-dependent. It was suggested that the effect of Gd on bone metabolism was complicated, and concentration and culture time were key factors for switching the biological effects of Gd from damage to protection.
张金超，李亚平，张群，郝晓红，王书香
Chemical Biology Key Laboratory of Hebei Province, College of Chemistry and Environmental Science, Hebei University
摘 要：To evaluate the effects of Gd on proliferation, differentiation and mineralization function of primary osteoblasts （OBs） in vitro, we tested cell viability by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay, cell differentiation by alkaline phosphatase （ALP） activity assay, synthesis of type ? collagen, and oil red O and alizarin red S （ARS） stain assays. The results indicated that effects of Gd on the proliferation, osteogenic differentiation, mineralization function and adipocytic transdifferentiation of primary OBs de-pended on concentration and incubation time, but were not dose-dependent. It was suggested that the effect of Gd on bone metabolism was complicated, and concentration and culture time were key factors for switching the biological effects of Gd from damage to protection.
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