Effect of CO2 and N2 on microbial community changes during column bioleaching of low-grade high pyrite-bearing chalcocite ore
来源期刊：中南大学学报(英文版)2015年第12期
论文作者：CHEN Bo-wei WU Biao LIU Xing-yu WEN Jian-kang
文章页码：4528 - 4535
Key words：bioleaching; CO2; N2; chalcocite; pyrite; microbial community
Abstract: Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40 °C with aeration of CO2 and N2. Ore samples were collected at day 43, 64, 85, 106 and subjected to microbial community analysis by 16S rRNA gene clone library. Phylogenetic analyses of 16S rDNA fragments revealed that the retrieved sequences are mainly related to genus Acidithiobacillus, Leptospirillum and Sulfobacillus. Aeration of CO2 and N2 significantly impacted the microbial community composition. When CO2 was aerated, the proportion of genus Acidithiobacillus considerably increased, whereas the proportion of genus Leptospirillum and genus Sulfobacillus declined. However, with the aeration of N2, the proportion of genus Acidithiobacillus and Leptospirillum increased, but genus Sulfobacillus decreased. When there was no aeration, the microbial community was similar to the inocula with the proportion of genus Leptospirillum mounted. These results indicated that the limitation of oxygen could change the bioleaching microbial community and the aeration of CO2 and N2 was favourable for the growth of sulfur-oxidizer (At. caldus) and iron-oxidizer (L. ferriphilum) respectively, which could be used for the regulation of microorganisms’ role in mineral bioleaching.
CHEN Bo-wei(陈勃伟), WU Biao(武彪), LIU Xing-yu(刘兴宇), WEN Jian-kang(温建康)
(National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals,
Beijing 100088, China)
Abstract:Simulated heap bioleaching of low-grade high pyrite-bearing chalcocite ore was conducted at 40 °C with aeration of CO2 and N2. Ore samples were collected at day 43, 64, 85, 106 and subjected to microbial community analysis by 16S rRNA gene clone library. Phylogenetic analyses of 16S rDNA fragments revealed that the retrieved sequences are mainly related to genus Acidithiobacillus, Leptospirillum and Sulfobacillus. Aeration of CO2 and N2 significantly impacted the microbial community composition. When CO2 was aerated, the proportion of genus Acidithiobacillus considerably increased, whereas the proportion of genus Leptospirillum and genus Sulfobacillus declined. However, with the aeration of N2, the proportion of genus Acidithiobacillus and Leptospirillum increased, but genus Sulfobacillus decreased. When there was no aeration, the microbial community was similar to the inocula with the proportion of genus Leptospirillum mounted. These results indicated that the limitation of oxygen could change the bioleaching microbial community and the aeration of CO2 and N2 was favourable for the growth of sulfur-oxidizer (At. caldus) and iron-oxidizer (L. ferriphilum) respectively, which could be used for the regulation of microorganisms’ role in mineral bioleaching.
Key words:bioleaching; CO2; N2; chalcocite; pyrite; microbial community
