Synthesis and Applications of Tetra- functional Branched Poly（ethylene glycol） Derivative for the Decellularized Valve Leaflets Cross-linking
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2015年第1期
论文作者：胡行健 董念国 SHI Jiawei LI Huadong DENG Cheng LU Cuifen
文章页码：193 - 197
摘 要：To investigate the effects of polyethylene glycol cross-linking on the mechanical properties, 80 porcine aortic valves were harvested, decellularized, and introduced with sulfhydryl. Then the valves were randomly assigned into 5 experimental groups and 1 control group（n=16）. For the valves in those experimental groups, branched polyethylene glycol diacrylate（PEG） of 5 different molecular weights（3.4, 8, 12, 20, 40 k Da） were synthesized and cross-linked with them respectively. The efficiency of the cross-linking was determined by measuring the amount of residual thiol group and the mechanical properties of the cross-linked valve leaflets were assessed by uni-axial planar tensile testing. The efficiency of the PEG 20 k Da group was 70.72±2.33%, obviously superior to that of the other groups（p<0.05）. Tensile test proved that branched PEG cross-linking can significantly enhance the mechanical behaviors of the decellularized valve leaflet and the Young’s modulus of each group was positively correlated with the molecular weight of PEG. It was concluded that branched PEG with the molecular weight of 20 k Da can effectively cross-link the decellularized porcine aortic valves and improve their mechanical properties, which makes it a promising cross-linker that can be used in the modification of decellularized tissue engineering valves.
胡行健1，董念国1，SHI Jiawei1，LI Huadong1，DENG Cheng1，LU Cuifen2
1. Department of Cardiovascular Surgery, Union Hospital, Huazhong University of Science and Technology2. School of Chemistry and Chemical Engineering, Hubei University
摘 要：To investigate the effects of polyethylene glycol cross-linking on the mechanical properties, 80 porcine aortic valves were harvested, decellularized, and introduced with sulfhydryl. Then the valves were randomly assigned into 5 experimental groups and 1 control group（n=16）. For the valves in those experimental groups, branched polyethylene glycol diacrylate（PEG） of 5 different molecular weights（3.4, 8, 12, 20, 40 k Da） were synthesized and cross-linked with them respectively. The efficiency of the cross-linking was determined by measuring the amount of residual thiol group and the mechanical properties of the cross-linked valve leaflets were assessed by uni-axial planar tensile testing. The efficiency of the PEG 20 k Da group was 70.72±2.33%, obviously superior to that of the other groups（p<0.05）. Tensile test proved that branched PEG cross-linking can significantly enhance the mechanical behaviors of the decellularized valve leaflet and the Young’s modulus of each group was positively correlated with the molecular weight of PEG. It was concluded that branched PEG with the molecular weight of 20 k Da can effectively cross-link the decellularized porcine aortic valves and improve their mechanical properties, which makes it a promising cross-linker that can be used in the modification of decellularized tissue engineering valves.
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