A Novel Tissue Adhesive Composed of Modified Gelatin and Polysaccharides
来源期刊：材料科学与工程学报2000年增刊第2期
论文作者：Hiroo IWATA Yoshito Ikada Xiumei MO
关键词：Hemostatic agent; Gelatin; Polysaccharides; Gel; Dextran: Hydroxyethyl starch; Schiff's base: adhesive;
摘 要：A two-component adhesive glue was prepared from gelatin and polysaccharides. Gelatin was modified with ethylenediamine (ED) in the presence of water soluble carbodiimine (WSC) to introduce more amino groups into the original gelatin, while dextran and hydroxyethyl-starch (HES) were oxidized by sodium periodate to convert neighbouring hydroxyl groups into dialdehyde groups. Upon mixing the two polymer components in aqueous solution, Schiff's base reaction occurred between the amino groups in the modified gelatin and the aldehyde groups in the modified polysaccharides to give intermolecular crosslinking and gel formation. This new glue showed a capability to stop bleeding and seal tissues when a cut liver of rat was used for hemostatic experiments. It could also form a gel much faster than fibrin glue in in-vitro experiments. The fastest gel formation took place within 2 sec and the bonding strength to porcine skin was about 225gf/cm2 when an amino-gelatin (55% amino) and an aldehyde-HES (>84% dialdehyde) was mixed. In contrast, the gelation time and bonding strength of fibrin gluewere 5 seconds and 120gf/cm2, respectively. The effective adhesive properties have also been identified by the animal experiments of rat.
Hiroo IWATA1，Yoshito Ikada1，Xiumei MO1
(1.Institute for Frontier Medical Science, Kyoto University, 53 Kawahara-cho, Sakyo-ku, Kyoto, Japan;
2.Institute of Materials Science, East China University of Science and Technology,Shanghai 200237, China)
摘要:A two-component adhesive glue was prepared from gelatin and polysaccharides. Gelatin was modified with ethylenediamine (ED) in the presence of water soluble carbodiimine (WSC) to introduce more amino groups into the original gelatin, while dextran and hydroxyethyl-starch (HES) were oxidized by sodium periodate to convert neighbouring hydroxyl groups into dialdehyde groups. Upon mixing the two polymer components in aqueous solution, Schiff''s base reaction occurred between the amino groups in the modified gelatin and the aldehyde groups in the modified polysaccharides to give intermolecular crosslinking and gel formation. This new glue showed a capability to stop bleeding and seal tissues when a cut liver of rat was used for hemostatic experiments. It could also form a gel much faster than fibrin glue in in-vitro experiments. The fastest gel formation took place within 2 sec and the bonding strength to porcine skin was about 225gf/cm2 when an amino-gelatin (55% amino) and an aldehyde-HES (>84% dialdehyde) was mixed. In contrast, the gelation time and bonding strength of fibrin gluewere 5 seconds and 120gf/cm2, respectively. The effective adhesive properties have also been identified by the animal experiments of rat.
关键词:Hemostatic agent; Gelatin; Polysaccharides; Gel; Dextran: Hydroxyethyl starch; Schiff''s base: adhesive;
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