Effect of Hydroxyapatitc Nanoparticles on K562 Cells in vitro

CHEN Pei~(1,2) DAI Honglian~1 HAN Yingchao~1 YIN Meizhen~1 LI Shipu~1 (1.Biomedical Materials and Engineering Center,Wuhan University of Technology,Wuhan,430070,China; 2.Nursing school,Wuhan Railway Vocational College of Technology,Wuhan,430205,China)

摘 要：<正>Stable and single-dispersed hydroxyapatite（HAP）nanoparticles were synthesized with ultrasonic-assisted method.HAP nanopanicles were characterized by dynamic light scattering,XRD（X-ray diffraction）and TEM（Transmission Electron Microscopy）.The effect of HAP nanopanicles on the K562 human myelogenous leukemia cell line was investigated by MTT assay and cell count test,and the mechanism was studied through the changes of cell cycle and ultrastructure.The results showed that HAP nanoparticles inhibited the proliferation of K562 cells dramatically in vitro.HAP nanoparticles entered the cytoplasm of K562 cells and the cells were arrested at G₂/M phase,thus,the cells died directly.

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