百合中秋水仙碱的分离及结构表征

来源期刊：中南大学学报(自然科学版)2006年第2期

论文作者：何纯莲 任凤莲 吴泓毅 陈蓉

文章页码：286 - 289

关键词：百合;秋水仙碱;超临界流体;萃取;分离

Key words：lily; colchicine; supercritical fluid; extraction; separation

摘    要：采用超临界二氧化碳流体萃取百合中秋水仙碱,用高效液相色谱法对萃取物中秋水仙碱的含量进行测定,用重结晶、薄层色谱法对萃取物中秋水仙碱进行分离纯化,纯化后的目标物用核磁共振和红外光谱进行表征。研究结果表明:采用高效液相色谱法测得萃取物中秋水仙碱的质量分数为6.4%,纯化后分离收率为88%,核磁共振和红外光谱表征的结果与标准物的图谱一致,可见超临界流体萃取—测定—分离百合中秋水仙碱的方法具有较好的选择性。

Abstract: The colchicine content of lily in supercritical carbon dioxide fluid extraction was determined by high performance liquid chromatography. Pure colchicine was separated from the extracts through thin-layer chromatography and was identified by using spectral techniques of nuclear magnetic resonance and infrared spectrum. The results show that colchicine content is increased to 6.4% in extract. The recovery of colchicine is 88%, and spectral diagram of colchicine from lily is the same as that of colchicine standard sample. The method of extraction-detection-separation colchicine from lily is applicable and it has a preferable selectivity.