Lanthanum Chloride Inhibiting Expression of Inducible Nitric Oxide Synthase in RAW264.7 Macrophages Induced by Lipopolysaccharide
来源期刊：JOURNAL OF RARE EARTHS2007年第3期
论文作者：Xie An Lou Yuanlei Wang Yang Guo Fei Li Guohui
Key words：lanthanum chloride; lipopolysaccharide; nitric oxide; inducible nitric oxide synthase; rare earths;
Abstract: Nitric oxide (NO) and its reaction products were key players in the pathophysiology of sepsis and shock. The present study was designed to explore the effects of lanthanum chloride (LaCl3) on inducible nitric oxide synthase (iNOS) expression, at both gene and protein levels, in RAW264.7 macrophages induced by Lipopolysaccharide (LPS). Reverse transcription polymerase chain reaction (RT-PCR), immunofluorescence, and western blot were employed to measure iNOS gene expression, localization, and protein expression respectively. NO production in culture supernatants was detected by the nitrate reductase method. The results showed that LaCl3 significantly attenuated the iNOS gene and protein expression, as well as NO production in RAW264.7cells induced by LPS.
Xie An1，Lou Yuanlei1，Wang Yang1，Guo Fei3，Li Guohui3
(1.Institute of Urology, Nanchang University, Nanchang 330006, China;
2.Postgraduate Department, Nanchang University, Nanchang 330006, China;
3.Burns Institute, Nanchang University , Nanchang 330006, China)
Abstract:Nitric oxide (NO) and its reaction products were key players in the pathophysiology of sepsis and shock. The present study was designed to explore the effects of lanthanum chloride (LaCl3) on inducible nitric oxide synthase (iNOS) expression, at both gene and protein levels, in RAW264.7 macrophages induced by Lipopolysaccharide (LPS). Reverse transcription polymerase chain reaction (RT-PCR), immunofluorescence, and western blot were employed to measure iNOS gene expression, localization, and protein expression respectively. NO production in culture supernatants was detected by the nitrate reductase method. The results showed that LaCl3 significantly attenuated the iNOS gene and protein expression, as well as NO production in RAW264.7cells induced by LPS.
Key words:lanthanum chloride; lipopolysaccharide; nitric oxide; inducible nitric oxide synthase; rare earths;
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