Folic Acid Functionalized Vertically Aligned Carbon Nanotube（FA-VACNT） Electrodes for Cancer Sensing Applications
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2016年第7期
论文作者：Somayeh Zanganeh Fatemeh Khodadadei S.Ra?zadeh Tafti Mohammad Abdolahad
文章页码：617 - 625
摘 要：An electrical cancer biosensor was developed using amine-functionalized vertically aligned carbon nanotubes（VACNTs） conjugated to folic acid（FA） molecules. Speci?c binding of FA to folate receptor（FR） existing on the membrane of cancer cells assisted their entrapment on VACNTs. For the conjugation of FA to CNTs,amine（—NH2） functional groups were attached to the side walls of the nanotubes by plasma treatment.The amount and shape of entrapped cancer cells on FA-VACNTs were noticeably higher and more uniform than the cells entrapped on bare VACNTs. The comparative signal spike of the FA-VACNTs and VACNTs covered impedance sensor in interaction with the same concentration of lung cancer cells（QUDB） showed sharper response for the functionalized sensor. Moreover, electron microscopy and ?orescent images as well as impedance diagrams veri?ed the spherical and non-deformed shape of the cells entrapped by FA-VACNT. This sensor would be useful in assaying the cells vitality in time evolution. This device could be applied in diagnostic and time monitoring applications in the ?eld of cancer such as extreme drug resistance assay（EDR）.
Somayeh Zanganeh1,2，Fatemeh Khodadadei1,2,3，S.Ra?zadeh Tafti1,2，Mohammad Abdolahad1,2
1. Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran2. Nanoelectronic Center of Excellence, Thin Film and Nanoelectronic Lab, School of Electrical and Computer Engineering, University of Tehran3. School of Chemical Engineering, College of Engineering, University of Tehran
摘 要：An electrical cancer biosensor was developed using amine-functionalized vertically aligned carbon nanotubes（VACNTs） conjugated to folic acid（FA） molecules. Speci?c binding of FA to folate receptor（FR） existing on the membrane of cancer cells assisted their entrapment on VACNTs. For the conjugation of FA to CNTs,amine（—NH2） functional groups were attached to the side walls of the nanotubes by plasma treatment.The amount and shape of entrapped cancer cells on FA-VACNTs were noticeably higher and more uniform than the cells entrapped on bare VACNTs. The comparative signal spike of the FA-VACNTs and VACNTs covered impedance sensor in interaction with the same concentration of lung cancer cells（QUDB） showed sharper response for the functionalized sensor. Moreover, electron microscopy and ?orescent images as well as impedance diagrams veri?ed the spherical and non-deformed shape of the cells entrapped by FA-VACNT. This sensor would be useful in assaying the cells vitality in time evolution. This device could be applied in diagnostic and time monitoring applications in the ?eld of cancer such as extreme drug resistance assay（EDR）.
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