Simultaneous determination of geniposidic acid，chlorogenic acid and geniposide in eucommia by HPLC

来源期刊：中南大学学报(英文版)2003年第3期

论文作者：李宇萍 陈晓青 彭密军 贺前峰 出山武

文章页码：198 - 201

Key words：eucommia; HPLC; geniposidic acid; chlorogenic acid; geniposide

Abstract: A high performance liquid chromatography(HPLC) method was established for simultaneous determination of geniposidic acid, chlorogenic acid and geniposide in eucommia. Detection at 240 nm with a reversed-phase column, CH₃OH volume fraction, acidic additive and pH value of mobile phase were studied for their effects on the separability of the compounds. The most suitable separation was obtained with isocratic gradient elution system using CH₃OH-H₂O-H₃P0₄<12.00，87. 96，0. 04，volume ratio) at a flow-rate of 1. 0 mL/min. Under the experimental conditions, the capacity factors of three compounds are in 3-13. The sample is separated rightly. The analysis time is 30 min and the retention time of geniposidic acid, chlorogenic acid and geniposide are 6. 7 min, 10. 5 min and 21 min, respectively.