Screening, identification and desilication of a silicate bacterium①
来源期刊：中南大学学报(英文版)2006年第4期
论文作者：周洪波 曾晓希 刘飞飞 邱冠周 胡岳华
文章页码：337 - 341
Key words：screening; identification;Bacillus mucilaginosus; silicon removal
Abstract: The strain Lv1-2 isolated from the Henan bauxite was characterized by morphological observation, biochemical and physiological identification, and 16S rDNA sequence analysis. The influences of temperature, initial pH value, the volume of medium, shaking speed and illite concentration on the desilicating ability of the strain Lv1-2 were investigated. The results show that the bacterium is a Gram-negative rod-shaped bacterium with oval endspores and thick capsule, but without flagellum. The biochemical and physiological tests indicate that the strain Lv1-2 is similar toBacillus mucilaginosus. In GenBank the 16S rDNA sequence similarity of the strain Lv1-2 and theB.mucilaginosusYNUCC0001(AY571332) is more than 99%. Based on the above results, the strain Lv1-2 is identified as B.mucilaginosus. The optimum conditions for the strain Lv1-2 to remove silicon from illite are as follows: temperature is 30℃;initial pH value is 7.5; medium volume in 200 mL bottle is 60 mL; shaking speed of rotary shaker is 220 r/m ; illite concentration is 1%.
基金信息：the National Natural Science Foundation of China
