Biochemical effects of gadolinium chloride in rats liver and kidney studied by 1H NMR metabolomics
来源期刊：JOURNAL OF RARE EARTHS2009年第2期
论文作者：WU Yijie LI Weisheng LI Xiaojing LIAO Peiqiu PEI Fengkui WEI Lai NI Jiazuan Wu Huifeng
Key words：gadolinium chloride; NMR; liver; kidney; metaboiomics; tissue extracts; rat; rare earths;
Abstract: The biochemical effects of gadolinium chloride were studied using high-resolution IH nuclear magnetic resonance (NMR) spec-troscopy to investigate the biochemical composition of tissue (liver and kidney) aqueous extracts obtained from control and gadolinium chlo-ride (GdCl3) (10 and 50 mg/kg body weight, intraperitoneal injection, i.p.) treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCl3, and extracted using methanol/chloroform solvent system. 1H NMR spectra of tissue extracts were analyzed by pat-tern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and de-creased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine (GPC), betaine, myo-inositoi and trimethylamine N-oxide (TMAO)levels in kidney illustrated kidney disturbance induced by GdCl3.
WU Yijie1，LI Weisheng1，LI Xiaojing1，LIAO Peiqiu1，PEI Fengkui1，WEI Lai1，NI Jiazuan1，Wu Huifeng1
(1.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;
2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China)
Abstract:The biochemical effects of gadolinium chloride were studied using high-resolution IH nuclear magnetic resonance (NMR) spec-troscopy to investigate the biochemical composition of tissue (liver and kidney) aqueous extracts obtained from control and gadolinium chlo-ride (GdCl3) (10 and 50 mg/kg body weight, intraperitoneal injection, i.p.) treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCl3, and extracted using methanol/chloroform solvent system. 1H NMR spectra of tissue extracts were analyzed by pat-tern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and de-creased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine (GPC), betaine, myo-inositoi and trimethylamine N-oxide (TMAO)levels in kidney illustrated kidney disturbance induced by GdCl3.
Key words:gadolinium chloride; NMR; liver; kidney; metaboiomics; tissue extracts; rat; rare earths;
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