Synthesis and characterization of NaYF₄:Yb³⁺,Er³⁺@silica-N=folic acid nanocomplex for bioimaginable detecting MCF-7 breast cancer cells

Ha Thi Phuong^1,2，Tran Thu Huong^1,3，Le Thi Vinh⁴，Hoang Thi Khuyen^1,3，Do Thi Thao^3,5，Nguyen Thanh Huong^1,3，Pham Thi Lien^1,3，Le Quoc Minh^1,6

1. Institute of Materials Science,Vietnam Academy of Science and Technology3. Graduate University of Science and Technology-Vietnam Academy of Science and Technology4. Department of Chemistry,Hanoi University of Mining and Geology5. Institute of Biotechnology,Vietnam Academy of Science and Technology6. Duy Tan University

摘 要：Upconversion nanophosphors are new promising nanomaterials to be used as biolabels for detection and imaging of cancer cells.These nanophosphors absorb long-wavelength excitation radiation in the infrared or near infrared region and emit shorter wavelength,higher energy radiation from ultraviolet to infrared.In this paper,we studied the hydrothermal method and optical properties of the functionalized NaYF₄:Yb³⁺,Er³⁺for biomedical application.After synthesis,these NaYF₄:Yb³⁺,Er³⁺nanophosphors were functionalized with aminosilanes and folic acid.Folic acid binds to the folate receptor on the surface of MCF-7 breast cancer cells and this binding promotes internalization of the nanophosphors via endocytosis.The sizes of the functionalized NaYF₄:Yb³⁺,Er³⁺@silica-N=FA（folic acid） nanophosphors can be controlled with length of the rod about 300-800 nm and diameter of the rod about 100-200 nm.Phase structure of NaYF₄:Yb³⁺,Er³⁺is in hexagonal crystal system.The photo luminescence（PL） spectra of the functionalized NaYF₄:Yb³⁺,Er³⁺@silica-N=FA nanophosphors were measured.These nanophosphors emit in red color with the strongest band at 650 nm under 980 nm excitation.This result can provide NaYF₄:Er³⁺,Yb³⁺@silica-N=FA complex for developing fluorescence label and image tool in cancer biology and medicine.

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