中度嗜热混合菌在搅拌槽中浸出黄铜矿及其群落动态

来源期刊:中南大学学报(自然科学版)2010年第1期

论文作者:周洪波 谢英剑 张汝兵 曾伟民 罗海浪 王玉光

文章页码:15 - 20

关键词:黄铜矿精矿;槽式搅拌反应器;中度嗜热菌;PCR-RFLP;群落变化

Key words:chalcopyrite concentrate; stirred tank bioreactor; moderately thermophilic microorganism; PCR-RFLP;community succession

摘    要:为了优化浸出工艺,研究浸矿体系中主要微生物种群的结构和动态变化,在槽式搅拌反应器中于45℃用中度嗜热混合菌(Acidithiobacillus caldus,Leptospirillum ferriphilum,Sulfobacillus acidophilus,Sulfobacillus thermosulfidooxidans等)浸出黄铜矿精矿,考察浸出液的pH,Eh及金属离子浓度并应用PCR-RFLP(限制性酶切片段长度多态性)方法研究细菌群落变化,浸出时间为30 d。研究结果表明:Cu的浸出率为26.2%,反应器内菌种多样性不丰富,只有At. caldus 和L. ferriphilum 2种;群落动态变化明显,在浸出开始阶段At. caldus 是优势种群,其丰度为96%,随着浸出的进行,L. ferriphilum 逐渐增多,在浸出后期代替At. caldus 成为优势种群,其丰度为69%。

Abstract: Community succession in bioleaching process was investigated for optimization. Chalcopyrite concentrate was leached by moderately thermophilic mixed microorganisms, including Acidithiobacillus caldus, Leptospirillum ferriphilum, Sulfobacillus acidophilus, Sulfobacillus thermosulfidooxidans et al. in a stirred tank bioreactor operated at 45 ℃ for 30 d. The parameters such as pH, Eh and concentrations of metal ions were determined and synchronously the method of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was used to analyze the succession of bacterial community, and their possible influences on succession of community were discussed. The results show that copper recovery is 26.2%. Only two species, i.e., At. caldus and L. ferriphilum are detected. At the beginning of bioleaching process, At. caldus is the predominant species, accounting for 96% of the whole community. During the process of leaching, L. ferriphilum gradually increases and replaces At. caldus, and accounts for 69% as the majority of community in the later stage of bioleaching process.

基金信息:国际海底区域研究开发“十一五”项目
海洋公益性行业科研专项经费资助项目
教育部新世纪优秀人才支持计划项目

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