Thin-layer heap bioleaching of copper flotation tailings containing high levels of fine grains and microbial community succession analysis
来源期刊：International Journal of Minerals Metallurgy and Materials2017年第4期
论文作者：Xiao-dong Hao Yi-li Liang Hua-qun Yin Hong-wei Liu Wei-min Zeng Xue-duan Liu
文章页码：360 - 368
摘 要：Thin-layer heap bioleaching of copper flotation tailings containing high levels of fine grains was carried out by mixed cultures on a small scale over a period of 210 d. Lump ores as a framework were loaded at the bottom of the ore heap. The overall copper leaching rates of tailings and lump ores were 57.10wt% and 65.52wt%,respectively. The dynamic shifts of microbial community structures about attached microorganisms were determined using the Illumina Mi Seq sequencing platform based on 16 S rRNA amplification strategy. The results indicated that chemolithotrophic genera Acidithiobacillus and Leptospirillum were always detected and dominated the microbial community in the initial and middle stages of the heap bioleaching process; both genera might be responsible for improving the copper extraction. However,Thermogymnomonas and Ferroplasma increased gradually in the final stage. Moreover,the effects of various physicochemical parameters and microbial community shifts on the leaching efficiency were further investigated and these associations provided some important clues for facilitating the effective application of bioleaching.
Xiao-dong Hao1,2，Yi-li Liang1,2，Hua-qun Yin1,2，Hong-wei Liu1,2，Wei-min Zeng1,2，Xue-duan Liu1,2
1. School of Minerals Processing and Bioengineering,Central South University2. Key Laboratory of Biometallurgy of Ministry of Education,Central South University
摘 要：Thin-layer heap bioleaching of copper flotation tailings containing high levels of fine grains was carried out by mixed cultures on a small scale over a period of 210 d. Lump ores as a framework were loaded at the bottom of the ore heap. The overall copper leaching rates of tailings and lump ores were 57.10wt% and 65.52wt%,respectively. The dynamic shifts of microbial community structures about attached microorganisms were determined using the Illumina Mi Seq sequencing platform based on 16 S rRNA amplification strategy. The results indicated that chemolithotrophic genera Acidithiobacillus and Leptospirillum were always detected and dominated the microbial community in the initial and middle stages of the heap bioleaching process; both genera might be responsible for improving the copper extraction. However,Thermogymnomonas and Ferroplasma increased gradually in the final stage. Moreover,the effects of various physicochemical parameters and microbial community shifts on the leaching efficiency were further investigated and these associations provided some important clues for facilitating the effective application of bioleaching.
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