Isolation and characterization of YNTC-1,a novel Alicyclobacillus sendaiensis strain
来源期刊：中南大学学报(英文版)2008年第4期
论文作者：丁建南 何环 张成桂 于一尊 邱冠周
文章页码：508 - 514
Key words：bioleaching; sulphide minerals; isolation; strain YNTC-1; Alicyclobacillus sendaiensis; Sulfobacillus thermosulfidooxi- dans
Abstract:
A heterotrophic acidothermophilic bacterial strain, YNTC-1, was isolated from an acidic hot spring in Tengchong, Yunan, China. YNTC-1 grows at pH value of 1.5-8.0 and temperature of 40-70 ℃, with optimal pH and temperature at 3.0 and 55 ℃, respectively. The cells of the strain are in shape of short rod, with 1.0-1.2 μm in length and 0.7-0.8 μm in diameter, and with distinct spores at both poles of each cell. The predominant fatty acids in cellular membrane of the strain are C18:1 ω7c. 16s rRNA gene analysis reveals that this strain is closely related to Alicyclobacillus sendaiensis, with over 99% sequence similarity. Based on phenotypic and genotypic analyses, YNTC-1 is identified as a member of A. sendaiensis. Considering some important morphological and biochemical differences between strain YNTC-1 and A. sendaiensis ATCC 27009T, YNTC-1 may be proposed to be a novel subspecies of A. sendaiensis. However, this viewpoint has to be confirmed by further studies. Co-bioleaching of pyrite and chalcopyrite with strain YN22, Sulfobacillus thermosulfidooxidans, shows that strain YNTC-1 has no evident influence on bioleaching rates of these two sulphide minerals.
基金信息：the Chinese National Natural Science Foundation for Innovative Research Groups
the Major State Basic Research and Development Program of China
