Isolation of Leptospirillum ferriphilum by single-layered solid medium

来源期刊：中南大学学报(英文版)2007年第4期

论文作者：柳建设 谢学辉 肖升木 王秀美 赵文杰 田卓力

文章页码：467 - 467

Key words：Leptospirillum ferriphilum; isolation; purification; solid medium; bioleaching; concentrate chalcopyrite

Abstract: According to physiological and biochemical characteristics of Leptospirillum ferriphilum, a strain of object bacteria was isolated successfully. Bacteria were enriched by selective liquid medium and plated on designed single-layered agar solid medium. Colony was cultured and bacteria were collected. The morphologies of the object bacteria were observed using crystal violet staining, scanning electron microscope(SEM) and transmission electron microscope (TEM). The result of 16S rDNA identification shows that this bacterium belongs to Leptospirillum ferriphilum and it is named as Leptospirillum ferriphilum strain D1. These results indicate that this new single-layered agar solid medium is efficient and simple for isolation of Leptospirillum ferriphilum. Additionally, physiological-biochemical characteristics show that the optimum initial pH value and its growth temperature are 1.68 and 40 ℃, respectively. The culture of it is used to leach a complex concentrate chalcopyrite, the leaching efficiencies of copper and iron are 1.93 % and 13.74 %, respectively, and it is more effective than the A.ferrooxidans culture in the leaching of the complex concentrate chalcopyrite.

基金信息：the National Natural Science Foundation of China
the National Basic Research and Development Program of China
the Specialized Research Fund of the National; Excellent PhD Thesis