Comparison of microbial communities in three different mine drainages and their bioleaching efficiencies to low grade of chalcopyrite

来源期刊：中南大学学报(英文版)2007年第4期

论文作者：尹华群 邱冠周 王淀佐 曹琳辉 戴志敏 王杰伟 刘学端

文章页码：460 - 460

Key words：microbial community diversity; PCR-RFLP; principal component analysis; chalcopyrite; bioleaching

Abstract: Microbial community diversities in the drainage from three mines (Dexing Copper Mine, Qibaoshan Copper Mine and Yaogangxian Tungsten Mine, China) were analyzed using 16S rDNA PCR-RFLP approach. The efficiencies of chalcopyrite bioleaching were compared using enrichment of the three cultures. Phylogenetic analysis indicates that the dominant microorganisms are clustered with the Proteobacteria, the remaining is affiliated with Nitrospira, Acidobacteria and Actinobacteria. At the genus level, Acidithiobacillus is the dominant group in both YTW and QBS samples, while Spingomonas is dominant in YGX sample. Moreover, the principal component analysis (PCA) reveals that QBS and YTW have similar geochemical character and microbial communities. The results also show that pH value and tungsten concentration play a key role in microbial community distribution and relative abundance. The bioleaching efficiency of the enrichment cultures from YTW and QBS is similar. After 15 d, the bioleaching rates of low grade chalcopyrite (0.99%) are both up to 99.5% when using 10 g/L pulp density due to the similar microbial composition of YTW and QBS. Moreover, the leaching efficiencies of enrichment cultures containing multiple bioleaching microorganisms are higher than that of pure culture Acidithiobacillus ferrooxidans.

基金信息：the National Natural Science Foundation of China
the National Basic Research and Development Program of China