Characterization of tricalcium phosphate solubilization byStenotrophomonas maltophilia YC isolated from phosphate mines

来源期刊：中南大学学报(英文版)2009年第4期

论文作者：肖春桥 池汝安 何环 张文学

文章页码：581 - 588

Key words：tricalcium phosphate (TCP); Stenotrophomonas maltophilia (S. maltophilia); phosphate mines; phosphorus (P); gluconic acid

Abstract: The phosphate solubilizing characteristics of a strain YC, which was isolated from phosphate mines (Hubei, China), were studied in National Botanical Research Institute’s phosphate (NBRIP) growth medium containing tricalcium phosphate (TCP) as sole phosphorus (P) source. The strain YC is identified as Stenotrophomonas maltophilia (S. maltophilia) based upon the results of morphologic, physiological and biochemical characteristics and 16S rRNA sequences analysis. The results show that the strain S. maltophilia YC can solubilize TCP and release soluble P in NBRIP growth medium. A positive correlation between concentration of soluble P and population of the isolate and a negative correlation between concentration of soluble P and pH in the culture medium are observed from statistical analysis results. Moreover, gluconic acid is detected in the culture medium by HPLC analysis. It indicates that the isolate can release gluconic acid during the solubilizing experiment, which causes acidification of the culture medium and then TCP solubilization. S. maltophilia YC has a maximal TCP solubilizing capability when using maltose as carbon source and ammonium nitrate as nitrogen source, respectively, in NBRIP growth medium.

基金信息：the Major State Basic Research and Development Program of China
the Key Project Foundation of the Education Department of Hubei Province, China
the Open Research Fund of Key Laboratory for Green Chemical Process of Ministry of Education, China
the Youths Science Foundation of Wuhan Institute of Technology, China