一株Cr(Ⅵ)还原菌的鉴定及其还原特性

来源期刊：中南大学学报(自然科学版)2011年第2期

论文作者：柴立元 曾娟 苏艳蓉 杨志辉 杨卫春

文章页码：300 - 304

关键词：微生物；六价铬；还原；16S rDNA 中图分类号：Q89；X703 文献标志码：A

Key words：bacterium; hexavalent chromium; reduction; 16S rDNA

摘    要：从长沙铬盐厂区的被污染土壤中分离获得一株具高效还原高浓度Cr(Ⅵ)的细菌，利用PCR扩增获得该菌16S rDNA部分序列，经测序和比对确定菌株为Pannonibacter sp.。研究该菌株的生理特性、培养条件以及还原六价铬的最佳条件。研究结果表明，该菌为革兰氏阴性菌，显微镜下观察为短杆状，好氧；该菌在pH为9，温度为30 ℃时生长最好；pH为8，30 ℃时六价铬还原效果最佳；16 h内可将质量浓度为300 mg/L的Cr(Ⅵ)还原90%以上。

Abstract: One bacterium which can reduce high concentration of Cr(Ⅵ) was isolated from the soil seriously contaminated by chromium-containing slag in Changsha chromate factory. The bacterium was Gram-negative and short rod when observed using a microscope. The partial 16S rDNA sequence indicats that this strain is Pannonibacte sp, and is an aerobic bacterium with the optimal growth at pH 8 under 30 ℃ and the optimal Cr(Ⅵ) reduction at pH 9.0 under 30 ℃. Under the optimal conditions, more than 90% of 300 mg / L Cr (Ⅵ) is reduced by strain P in 16 h.

基金信息：国家杰出青年科学基金资助项目