Fluorescence spectroscopy characteristics of humic acid by inoculating white-rot fungus during different phases of agricultural waste composting

来源期刊：中南大学学报(英文版)2009年第3期

论文作者：黄红丽 曾光明 蒋荣清 袁兴中 喻曼 黄丹莲 张嘉超 冯冲凌

文章页码：440 - 443

Key words：composting; inoculation; Phanerochaete chrysosporium; humic acid; fluorescence spectroscopy

Abstract: The white-rot fungus, Phanerochaete chrysosporium (P. chrysosporium), was inoculated during different phases of agricultural waste composting, and its effect on the fluorescence spectroscopy characteristics of humic acid (HA) was studied. The results show that the emission spectra have a sharp peak at 400 nm and a broad shoulder with the maximum centered at 460 nm. The excitation spectra have two peaks and exhibit red shift (shift to longer wavelengths) at 470 nm. The synchronous scan spectra present a number of peaks and shoulders, and the peaks at shorter wavelengths disappear gradually and form a shoulder. At the final stage of composting, the fluorescence spectra have similar shapes, but the fluorescence intensities decrease. P. chrysosporium increases the degree of aromatization and polymerization of HA when it is inoculated during the second fermentation phase, while it does not produce an obvious change on the humification degree of HA when it is inoculated during the first fermentation phase. Compared with the fluorescence spectroscopy characteristics of HA from soil, the structure of HA from compost is simpler and the activity is higher.

基金信息：the Major State Basic Research and Development Program of China
the Program for Changjiang Scholars and Innovative Research Team in University of China
the National Natural Science Foundation of China
the Environmental Protection Technology Research Program of Hunan Province, China